› 1ouo

X-ray diffraction
2.3Å resolution

Crystal structure of the periplasmic endonuclease Vvn

Released:
DOI: 10.2210/pdb1ouo/pdb
PDB entry 1ouo coloured by chain, front view.
PDB entry 1ouo coloured by chain, side view.
PDB entry 1ouo coloured by chain, top view.
Source organism: Vibrio vulnificus
Primary publication:
DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site.
Li CL, Hor LI, Chang ZF, Tsai LC, Yang WZ, Yuan HS
EMBO J 22 4014-25 (2003)
PMID: 12881435

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181723 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease I Chain: A
Molecule details ›
Chain: A
Length: 210 amino acids
Theoretical weight: 24.7 KDa
Source organism: Vibrio vulnificus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7MHK3 (Residues: 19-228; Coverage: 99%)
Gene name: VV2866
Sequence domains: Endonuclease I

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand MSE 5 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-18B
Spacegroup: P21
Unit cell:
a: 49.115Å b: 40.099Å c: 50.631Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.186 0.186 0.269
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Disease modeling and stem cell immunoengineering in regenerative medicine using CRISPR/Cas9 systems.
Antao et al. (2020)
1 more

4 mentions without citation

DNA binding and cleavage by the periplasmic nuclease Vvn: a novel structure with a known active site.
Li et al. (2003)
3 more