› 1oy5

X-ray diffraction
2.6Å resolution

Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus

Released:
DOI: 10.2210/pdb1oy5/pdb
PDB entry 1oy5 coloured by chain, front view.
PDB entry 1oy5 coloured by chain, side view.
PDB entry 1oy5 coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystal structure of tRNA (m1G37) methyltransferase from Aquifex aeolicus at 2.6 A resolution: a novel methyltransferase fold.
Liu J, Wang W, Shin DH, Yokota H, Kim R, Kim SH
Proteins 53 326-8 (2003)
PMID: 14517984

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130495 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (guanine-N(1)-)-methyltransferase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 257 amino acids
Theoretical weight: 29.69 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67463 (Residues: 1-257; Coverage: 100%)
Gene names: aq_1489, trmD
Sequence domains: tRNA (Guanine-1)-methyltransferase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 153.351Å b: 96.139Å c: 57.426Å
α: 90° β: 96.24° γ: 90°
R-values:
R R work R free
0.28 0.279 0.3
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Methylated nucleosides in tRNA and tRNA methyltransferases.
Hori H. (2014)
4 more

4 mentions without citation

Structural and evolutionary bioinformatics of the SPOUT superfamily of methyltransferases.
Tkaczuk et al. (2007)
3 more