› 1oy7

X-ray diffraction
2.7Å resolution

Structure and Function Analysis of Peptide Antagonists of Melanoma Inhibitor of Apoptosis (ML-IAP)

Released:
DOI: 10.2210/pdb1oy7/pdb
PDB entry 1oy7 coloured by chain, front view.
PDB entry 1oy7 coloured by chain, side view.
PDB entry 1oy7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure and function analysis of peptide antagonists of melanoma inhibitor of apoptosis (ML-IAP).
Franklin MC, Kadkhodayan S, Ackerly H, Alexandru D, Distefano MD, Elliott LO, Flygare JA, Mausisa G, Okawa DC, Ong D, Vucic D, Deshayes K, Fairbrother WJ
Biochemistry 42 8223-31 (2003)
PMID: 12846571

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric
hetero dimer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188402 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Baculoviral IAP repeat-containing protein 7 30kDa subunit Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 140 amino acids
Theoretical weight: 15.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96CA5 (Residues: 63-179; Coverage: 39%)
Gene names: BIRC7, KIAP, LIVIN, MLIAP, RNF50, UNQ5800/PRO19607/PRO21344
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
AEVVAVKSE peptide Chain: F
Molecule details ›
Chain: F
Length: 9 amino acids
Theoretical weight: 932 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand ZN 5 x ZN
Ligand P33 1 x P33
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P32
Unit cell:
a: 83.878Å b: 83.878Å c: 94.34Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.155 0.152 0.211
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

20 review citations

Targeting IAP proteins for therapeutic intervention in cancer.
Fulda et al. (2012)
19 more