› 1oya

X-ray diffraction
2Å resolution

OLD YELLOW ENZYME AT 2 ANGSTROMS RESOLUTION: OVERALL STRUCTURE, LIGAND BINDING AND COMPARISON WITH RELATED FLAVOPROTEINS

Released:
DOI: 10.2210/pdb1oya/pdb
PDB entry 1oya coloured by chain, front view.
PDB entry 1oya coloured by chain, side view.
PDB entry 1oya coloured by chain, top view.
Source organism: Saccharomyces pastorianus
Primary publication:
Old yellow enzyme at 2 A resolution: overall structure, ligand binding, and comparison with related flavoproteins.
Fox KM, Karplus PA
Structure 2 1089-105 (1994)
PMID: 7881908

Function and Biology Details

Reaction catalysed: 
NADPH + acceptor = NADP(+) + reduced acceptor
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169781 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NADPH dehydrogenase 1 Chain: A
Molecule details ›
Chain: A
Length: 400 amino acids
Theoretical weight: 45.07 KDa
Source organism: Saccharomyces pastorianus
Expression system: Not provided
UniProt:
  • Canonical: Q02899 (Residues: 1-400; Coverage: 100%)
Gene name: OYE1
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 142.88Å b: 142.88Å c: 43.01Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.241
Expression system: Not provided

Quick links

Citations

8 review citations

Reduction of polynitroaromatic compounds: the bacterial nitroreductases.
Roldán et al. (2008)
7 more

9 mentions without citation

Functional characterization and stability improvement of a 'thermophilic-like' ene-reductase from Rhodococcus opacus 1CP.
Riedel et al. (2015)
8 more