› 1oz0

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF THE HOMODIMERIC BIFUNCTIONAL TRANSFORMYLASE AND CYCLOHYDROLASE ENZYME AVIAN ATIC IN COMPLEX WITH A MULTISUBSTRATE ADDUCT INHIBITOR BETA-DADF.

Released:
DOI: 10.2210/pdb1oz0/pdb
PDB entry 1oz0 coloured by chain, front view.
PDB entry 1oz0 coloured by chain, side view.
PDB entry 1oz0 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Structure of avian AICAR transformylase with a multisubstrate adduct inhibitor beta-DADF identifies the folate binding site.
Wolan DW, Greasley SE, Wall MJ, Benkovic SJ, Wilson IA
Biochemistry 42 10904-14 (2003)
PMID: 12974624

Function and Biology Details

Reactions catalysed: 
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151981 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional purine biosynthesis protein ATIC Chains: A, B
Molecule details ›
Chains: A, B
Length: 613 amino acids
Theoretical weight: 66.67 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31335 (Residues: 1-593; Coverage: 100%)
Gene names: ATIC, PURH
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand K 2 x K
Ligand PO4 2 x PO4
Ligand MS1 2 x MS1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P21
Unit cell:
a: 54.41Å b: 108.1Å c: 102.36Å
α: 90° β: 91.92° γ: 90°
R-values:
R R work R free
0.217 0.217 0.269
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Structural biology of the purine biosynthetic pathway.
Zhang et al. (2008)
2 more