› 1p14

X-ray diffraction
1.9Å resolution

Crystal structure of a catalytic-loop mutant of the insulin receptor tyrosine kinase

Released:
DOI: 10.2210/pdb1p14/pdb
PDB entry 1p14 coloured by chain, front view.
PDB entry 1p14 coloured by chain, side view.
PDB entry 1p14 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical evidence for an autoinhibitory role for tyrosine 984 in the juxtamembrane region of the insulin receptor.
Li S, Covino ND, Stein EG, Till JH, Hubbard SR
J Biol Chem 278 26007-14 (2003)
PMID: 12707268

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138931 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Insulin receptor subunit beta Chain: A
Molecule details ›
Chain: A
Length: 306 amino acids
Theoretical weight: 34.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P06213 (Residues: 1005-1310; Coverage: 23%)
Gene name: INSR
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 57.472Å b: 69.482Å c: 88.814Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.225 0.224 0.24
Expression system: Spodoptera frugiperda

Quick links

Citations

10 review citations

Juxtamembrane autoinhibition in receptor tyrosine kinases.
Hubbard SR. (2004)
9 more

5 mentions without citation

An integrated linkage, chromosome, and genome map for the yellow fever mosquito Aedes aegypti.
Timoshevskiy et al. (2013)
4 more