› 1p16

X-ray diffraction
2.7Å resolution

Structure of an mRNA capping enzyme bound to the phosphorylated carboxyl-terminal domain of RNA polymerase II

Released:
DOI: 10.2210/pdb1p16/pdb
PDB entry 1p16 coloured by chain, front view.
PDB entry 1p16 coloured by chain, side view.
PDB entry 1p16 coloured by chain, top view.
Source organism: Candida albicans
Primary publication:
Structure of an mRNA capping enzyme bound to the phosphorylated carboxy-terminal domain of RNA polymerase II.
Fabrega C, Shen V, Shuman S, Lima CD
Mol Cell 11 1549-61 (2003)
PMID: 12820968

Function and Biology Details

Reaction catalysed: 
GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160301 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
mRNA-capping enzyme subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 395 amino acids
Theoretical weight: 46.5 KDa
Source organism: Candida albicans
Expression system: Escherichia coli
UniProt:
  • Canonical: P78587 (Residues: 2-395; Coverage: 88%)
Gene name: CGT1
Sequence domains:
Structure domains:
phosphorylated peptide from C-terminal of RNA polymerase II Chains: C, D
Molecule details ›
Chains: C, D
Length: 21 amino acids
Theoretical weight: 2.42 KDa
Source organism: Candida albicans
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand PO4 3 x PO4
Ligand G 1 x G
Ligand GTP 1 x GTP
2 modified residues:
Ligand MSE 14 x MSE
Ligand SEP 6 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P21
Unit cell:
a: 47.112Å b: 124.97Å c: 92.705Å
α: 90° β: 100.54° γ: 90°
R-values:
R R work R free
0.2 0.2 0.266
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

37 review citations

Serine/threonine phosphatases: mechanism through structure.
Shi Y. (2009)
36 more

16 mentions without citation

Enzymology of RNA cap synthesis.
Ghosh et al. (2010)
15 more