› 1p1b

X-ray diffraction
2.8Å resolution

Guanidinoacetate methyltransferase

Released:
DOI: 10.2210/pdb1p1b/pdb
PDB entry 1p1b coloured by chain, front view.
PDB entry 1p1b coloured by chain, side view.
PDB entry 1p1b coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Monoclinic guanidinoacetate methyltransferase and gadolinium ion-binding characteristics.
Komoto J, Takata Y, Yamada T, Konishi K, Ogawa H, Gomi T, Fujioka M, Takusagawa F
Acta Crystallogr D Biol Crystallogr 59 1589-96 (2003)
PMID: 12925789

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanidinoacetate N-methyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 199 amino acids
Theoretical weight: 22.62 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10868 (Residues: 38-236; Coverage: 84%)
Gene name: Gamt
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 4 x SAH
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 54.15Å b: 162.37Å c: 55.7Å
α: 90° β: 96.4° γ: 90°
R-values:
R R work R free
0.229 0.214 0.269
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Guanidinoacetic acid supplementation in broiler chickens fed on corn-soybean diets affects performance in the finisher period and energy metabolites in breast muscle independent of diet nutrient density.
Majdeddin et al. (2018)
1 more

2 mentions without citation

Crystal Engineering of a Chiral Crystalline Sponge That Enables Absolute Structure Determination and Enantiomeric Separation.
Deng et al. (2023)
1 more