› 1p1c

X-ray diffraction
2.5Å resolution

Guanidinoacetate Methyltransferase with Gd ion

Released:
DOI: 10.2210/pdb1p1c/pdb
PDB entry 1p1c coloured by chain, front view.
PDB entry 1p1c coloured by chain, side view.
PDB entry 1p1c coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Monoclinic guanidinoacetate methyltransferase and gadolinium ion-binding characteristics.
Komoto J, Takata Y, Yamada T, Konishi K, Ogawa H, Gomi T, Fujioka M, Takusagawa F
Acta Crystallogr D Biol Crystallogr 59 1589-96 (2003)
PMID: 12925789

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanidinoacetate = S-adenosyl-L-homocysteine + creatine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145504 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanidinoacetate N-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 199 amino acids
Theoretical weight: 22.62 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10868 (Residues: 38-236; Coverage: 84%)
Gene name: Gamt
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAH 2 x SAH
1 bound ligand:
Ligand GD3 1 x GD3
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43
Unit cell:
a: 54.22Å b: 54.22Å c: 156.27Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.193 0.288
Expression system: Escherichia coli

Quick links

Citations

2 citation in other articles

Guanidinoacetic acid supplementation in broiler chickens fed on corn-soybean diets affects performance in the finisher period and energy metabolites in breast muscle independent of diet nutrient density.
Majdeddin et al. (2018)
1 more