› 1p1k

X-ray diffraction
2.1Å resolution

Crystal structure of the 1L-myo-inositol 1-phosphate synthase complexed with NADH in the presence of EDTA

Released:
DOI: 10.2210/pdb1p1k/pdb
PDB entry 1p1k coloured by chain, front view.
PDB entry 1p1k coloured by chain, side view.
PDB entry 1p1k coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structures of NAD(+)- and NADH-bound 1-l-myo-inositol 1-phosphate synthase.
Jin X, Geiger JH
Acta Crystallogr D Biol Crystallogr 59 1154-64 (2003)
PMID: 12832758

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146022 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-3-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 533 amino acids
Theoretical weight: 59.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P11986 (Residues: 1-533; Coverage: 100%)
Gene names: INO1, J0610, YJL153C
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 2 x NAI
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 151.978Å b: 97.613Å c: 121.717Å
α: 90° β: 126.15° γ: 90°
R-values:
R R work R free
0.227 0.209 0.261
Expression system: Escherichia coli

Quick links

Citations

10 citation in other articles

An insight into the molecular basis of salt tolerance of L-myo-inositol 1-P synthase (PcINO1) from Porteresia coarctata (Roxb.) Tateoka, a halophytic wild rice.
Ghosh Dastidar et al. (2006)
9 more