› 1p2e

X-ray diffraction
2.2Å resolution

H61A mutant of flavocytochrome c3

Released:
DOI: 10.2210/pdb1p2e/pdb
PDB entry 1p2e coloured by chain, front view.
PDB entry 1p2e coloured by chain, side view.
PDB entry 1p2e coloured by chain, top view.
Source organism: Shewanella frigidimarina
Primary publication:
Histidine 61: an important heme ligand in the soluble fumarate reductase from Shewanella frigidimarina.
Rothery EL, Mowat CG, Miles CS, Walkinshaw MD, Reid GA, Chapman SK
Biochemistry 42 13160-9 (2003)
PMID: 14609326

Function and Biology Details

Reaction catalysed: 
Succinate + a quinone = fumarate + a quinol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142991 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fumarate reductase (cytochrome) Chain: A
Molecule details ›
Chain: A
Length: 571 amino acids
Theoretical weight: 60.56 KDa
Source organism: Shewanella frigidimarina
Expression system: Shewanella frigidimarina
UniProt:
  • Canonical: P0C278 (Residues: 1-571; Coverage: 100%)
Gene names: fcc3, fccA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 4 x HEM

Cofactor: Ligand FAD 1 x FAD
3 bound ligands:
Ligand NA 1 x NA
Ligand FUM 1 x FUM
Ligand ACY 1 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P21
Unit cell:
a: 45.351Å b: 87.079Å c: 79.678Å
α: 90° β: 109.38° γ: 90°
R-values:
R R work R free
0.175 0.175 0.258
Expression system: Shewanella frigidimarina

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Citations

1 review citation

Catalytic mechanisms of complex II enzymes: a structural perspective.
Iverson TM. (2013)
6 other articles