› 1p3d

X-ray diffraction
1.7Å resolution

Crystal Structure of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) in Complex with UMA and ANP.

Released:
DOI: 10.2210/pdb1p3d/pdb
PDB entry 1p3d coloured by chain, front view.
PDB entry 1p3d coloured by chain, side view.
PDB entry 1p3d coloured by chain, top view.
Source organism: Haemophilus influenzae
Primary publication:
Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae.
Mol CD, Brooun A, Dougan DR, Hilgers MT, Tari LW, Wijnands RA, Knuth MW, McRee DE, Swanson RV
J Bacteriol 185 4152-62 (2003)
PMID: 12837790

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetyl-alpha-D-muramate + L-alanine = ADP + phosphate + UDP-N-acetyl-alpha-D-muramoyl-L-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155243 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramate--L-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 52.76 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P45066 (Residues: 1-475; Coverage: 100%)
Gene names: HI_1139, murC
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MN 4 x MN
Ligand UMA 2 x UMA
Ligand ANP 2 x ANP
1 modified residue:
Ligand MSE 30 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 74.92Å b: 87.328Å c: 86.113Å
α: 90° β: 104.86° γ: 90°
R-values:
R R work R free
0.17 0.168 0.194
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
5 more

9 mentions without citation

Crystal structures of active fully assembled substrate- and product-bound complexes of UDP-N-acetylmuramic acid:L-alanine ligase (MurC) from Haemophilus influenzae.
Mol et al. (2003)
8 more