› 1p51

X-ray diffraction
2.5Å resolution

Anabaena HU-DNA cocrystal structure (AHU6)

Released:
DOI: 10.2210/pdb1p51/pdb
PDB entry 1p51 coloured by chain, front view.
PDB entry 1p51 coloured by chain, side view.
PDB entry 1p51 coloured by chain, top view.
Source organism: Anabaena sp.
Primary publication:
Flexible DNA bending in HU-DNA cocrystal structures.
Swinger KK, Lemberg KM, Zhang Y, Rice PA
EMBO J 22 3749-60 (2003)
PMID: 12853489

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114358 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA-binding protein HU Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 94 amino acids
Theoretical weight: 10.09 KDa
Source organism: Anabaena sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P05514 (Residues: 1-94; Coverage: 100%)
Gene names: asr3935, hanA, hup
Sequence domains: Bacterial DNA-binding protein
Structure domains: IHF-like DNA-binding proteins
5'-D(*GP*CP*AP*TP*AP*TP*CP*AP*AP*TP*TP*TP*GP*TP*TP*GP*CP*AP*T)-3' Chains: E, F, H, I
Molecule details ›
Chains: E, F, H, I
Length: 19 nucleotides
Theoretical weight: 5.81 KDa
Source organism: Anabaena sp.
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P21
Unit cell:
a: 37.646Å b: 107.836Å c: 83.464Å
α: 90° β: 92.25° γ: 90°
R-values:
R R work R free
0.291 0.286 0.338
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

24 review citations

IHF and HU: flexible architects of bent DNA.
Swinger et al. (2004)
23 more

17 mentions without citation

Studying protein-DNA interactions using atomic force microscopy.
Beckwitt et al. (2018)
16 more