› 1p6x

X-ray diffraction
2Å resolution

Crystal structure of EHV4-TK complexed with Thy and SO4

Released:
DOI: 10.2210/pdb1p6x/pdb
PDB entry 1p6x coloured by chain, front view.
PDB entry 1p6x coloured by chain, side view.
PDB entry 1p6x coloured by chain, top view.
Source organism: Equid alphaherpesvirus 4
Primary publication:
Structural basis for the dual thymidine and thymidylate kinase activity of herpes thymidine kinases.
Gardberg A, Shuvalova L, Monnerjahn C, Konrad M, Lavie A
Structure 11 1265-77 (2003)
PMID: 14527394

Function and Biology Details

Reaction catalysed: 
ATP + thymidine = ADP + thymidine 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-150228 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thymidine kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 334 amino acids
Theoretical weight: 37.01 KDa
Source organism: Equid alphaherpesvirus 4
Expression system: Escherichia coli
UniProt:
  • Canonical: P24425 (Residues: 23-352; Coverage: 94%)
Gene name: TK
Sequence domains: Thymidine kinase from herpesvirus
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand SO4 6 x SO4
Ligand THM 2 x THM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2221
Unit cell:
a: 111.65Å b: 121.55Å c: 118.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.187 0.237
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Carboranyl thymidine analogues for neutron capture therapy.
Tjarks et al. (2007)
16 other articles

2 mentions without citation

Dodging the crisis of folding proteins with knots.
SuĊ‚kowska et al. (2009)
1 more