› 1p7t

X-ray diffraction
1.95Å resolution

Structure of Escherichia coli malate synthase G:pyruvate:acetyl-Coenzyme A abortive ternary complex at 1.95 angstrom resolution

Released:
DOI: 10.2210/pdb1p7t/pdb
PDB entry 1p7t coloured by chain, front view.
PDB entry 1p7t coloured by chain, side view.
PDB entry 1p7t coloured by chain, top view.
Source organism: Escherichia coli str. K-12 substr. W3110
Primary publication:
Structure of the Escherichia coli malate synthase G:pyruvate:acetyl-coenzyme A abortive ternary complex at 1.95 A resolution.
Anstrom DM, Kallio K, Remington SJ
Protein Sci 12 1822-32 (2003)
PMID: 12930982

Function and Biology Details

Reaction catalysed: 
Acetyl-CoA + H(2)O + glyoxylate = (S)-malate + CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153394 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Malate synthase G Chain: A
Molecule details ›
Chain: A
Length: 731 amino acids
Theoretical weight: 81.65 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 1-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Sequence domains:
Structure domains:
Malate synthase G Chain: B
Molecule details ›
Chain: B
Length: 731 amino acids
Theoretical weight: 81.67 KDa
Source organism: Escherichia coli str. K-12 substr. W3110
Expression system: Escherichia coli
UniProt:
  • Canonical: P37330 (Residues: 1-723; Coverage: 100%)
Gene names: JW2943, b2976, glc, glcB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand ACO 2 x ACO
4 bound ligands:
Ligand MG 2 x MG
Ligand PYR 2 x PYR
Ligand PEG 1 x PEG
Ligand PG4 2 x PG4
1 modified residue:
Ligand CSO 3 x CSO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P212121
Unit cell:
a: 73.931Å b: 107.394Å c: 204.974Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.194 0.294
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Enzymes of the mevalonate pathway of isoprenoid biosynthesis.
Miziorko HM. (2011)
4 more

5 mentions without citation

The product complex of M. tuberculosis malate synthase revisited.
Anstrom et al. (2006)
4 more