› 1p91

X-ray diffraction
2.8Å resolution

Crystal Structure of RlmA(I) enzyme: 23S rRNA n1-G745 methyltransferase (NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET ER19)

Released:
DOI: 10.2210/pdb1p91/pdb
PDB entry 1p91 coloured by chain, front view.
PDB entry 1p91 coloured by chain, side view.
PDB entry 1p91 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site.
Das K, Acton T, Chiang Y, Shih L, Arnold E, Montelione GT
Proc Natl Acad Sci U S A 101 4041-6 (2004)
PMID: 14999102

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + guanine(745) in 23S rRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(745) in 23S rRNA
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153281 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
23S rRNA (guanine(745)-N(1))-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 30.79 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36999 (Residues: 1-269; Coverage: 100%)
Gene names: JW1811, b1822, rlmA, rrmA, yebH
Sequence domains:
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments


Cofactor: Ligand SAM 2 x SAM
2 bound ligands:
Ligand ZN 2 x ZN
Ligand SO4 5 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: I222
Unit cell:
a: 107.187Å b: 122.279Å c: 143.138Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.248 0.296
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Integrative computational modeling of protein interactions.
Rodrigues et al. (2014)
1 more

5 mentions without citation

Performance of ZDOCK and ZRANK in CAPRI rounds 13-19.
Hwang et al. (2010)
4 more