› 1pa9

X-ray diffraction
2Å resolution

Yersinia Protein-Tyrosine Phosphatase complexed with pNCS (Yop51,Pasteurella X,Ptpase,Yop51delta162) (Catalytic Domain, Residues 163-468) Mutant With Cys 235 Replaced By Arg (C235r)

Released:
DOI: 10.2210/pdb1pa9/pdb
PDB entry 1pa9 coloured by chain, front view.
PDB entry 1pa9 coloured by chain, side view.
PDB entry 1pa9 coloured by chain, top view.
Source organism: Yersinia enterocolitica
Primary publication:
Crystal structure of the Yersinia protein-tyrosine phosphatase YopH complexed with a specific small molecule inhibitor.
Sun JP, Wu L, Fedorov AA, Almo SC, Zhang ZY
J Biol Chem 278 33392-9 (2003)
PMID: 12810712

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147287 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase YopH Chain: A
Molecule details ›
Chain: A
Length: 284 amino acids
Theoretical weight: 31.25 KDa
Source organism: Yersinia enterocolitica
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P15273 (Residues: 185-468; Coverage: 61%)
Gene names: yop51, yopH
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily

Ligands and Environments

1 bound ligand:
Ligand CSN 1 x CSN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 49.36Å b: 55.9Å c: 97.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.226 0.243
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Yersinia outer proteins: Yops.
Trosky et al. (2008)
10 more

7 mentions without citation

Aryl vinyl sulfonates and sulfones as active site-directed and mechanism-based probes for protein tyrosine phosphatases.
Liu et al. (2008)
6 more