› 1pam

X-ray diffraction
1.8Å resolution

CYCLODEXTRIN GLUCANOTRANSFERASE

Released:
DOI: 10.2210/pdb1pam/pdb
PDB entry 1pam coloured by chain, front view.
PDB entry 1pam coloured by chain, side view.
PDB entry 1pam coloured by chain, top view.
Source organism: Bacillus sp. 1011
Primary publication:
X-ray structure of cyclodextrin glucanotransferase from alkalophilic Bacillus sp. 1011. Comparison of two independent molecules at 1.8 A resolution.
Harata K, Haga K, Nakamura A, Aoyagi M, Yamane K
Acta Crystallogr D Biol Crystallogr 52 1136-45 (1996)
PMID: 15299574

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138718 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclomaltodextrin glucanotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 686 amino acids
Theoretical weight: 75.23 KDa
Source organism: Bacillus sp. 1011
Expression system: Escherichia coli
UniProt:
  • Canonical: P05618 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR571
Spacegroup: P1
Unit cell:
a: 64.93Å b: 74.45Å c: 79.12Å
α: 85.2° β: 105° γ: 101°
R-values:
R R work R free
0.161 0.161 0.211
Expression system: Escherichia coli

Quick links

Citations

5 review citations

The carbohydrate-binding module family 20--diversity, structure, and function.
Christiansen et al. (2009)
4 more

6 mentions without citation

Comparative systems analysis of the secretome of the opportunistic pathogen Aspergillus fumigatus and other Aspergillus species.
Vivek-Ananth et al. (2018)
5 more