› 1ph7

X-ray diffraction
2.9Å resolution

CRYSTAL STRUCTURE OF THE OXYTRICHA NOVA TELOMERE END-BINDING PROTEIN COMPLEXED WITH NONCOGNATE SSDNA GGGGTTTTGIGG

Released:
DOI: 10.2210/pdb1ph7/pdb
PDB entry 1ph7 coloured by chain, front view.
PDB entry 1ph7 coloured by chain, side view.
PDB entry 1ph7 coloured by chain, top view.
Source organism: Sterkiella nova
Primary publication:
Nucleotide shuffling and ssDNA recognition in Oxytricha nova telomere end-binding protein complexes.
Theobald DL, Schultz SC
EMBO J 22 4314-24 (2003)
PMID: 12912928

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114384 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Telomere-binding protein subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 460 amino acids
Theoretical weight: 52.46 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P29549 (Residues: 36-495; Coverage: 93%)
Gene names: MAC-56A, MAC-56K, MAC-56S
Sequence domains: Telomeric single stranded DNA binding POT1/CDC13
Structure domains: Nucleic acid-binding proteins
Telomere-binding protein subunit beta Chain: B
Molecule details ›
Chain: B
Length: 216 amino acids
Theoretical weight: 24.49 KDa
Source organism: Sterkiella nova
Expression system: Escherichia coli
UniProt:
  • Canonical: P16458 (Residues: 9-224; Coverage: 56%)
Gene names: MAC-41A, MAC-41S
Sequence domains: Telomere-binding protein beta subunit (TEBP beta)
Structure domains: Telomere-binding Protein Beta Subunit; Chain
5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*GP*GP*GP*T)-3' Chains: G, H
Molecule details ›
Chains: G, H
Length: 13 nucleotides
Theoretical weight: 4.11 KDa
5'-D(*GP*GP*GP*GP*TP*TP*TP*TP*GP*IP*GP*G)-3' Chain: D
Molecule details ›
Chain: D
Length: 11 nucleotides
Theoretical weight: 3.46 KDa

Ligands and Environments

2 bound ligands:
Ligand NA 3 x NA
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: P6122
Unit cell:
a: 93.194Å b: 93.194Å c: 421.585Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.23 0.268
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Single-stranded DNA-binding proteins: multiple domains for multiple functions.
Dickey et al. (2013)
6 more

2 mentions without citation

Nucleotide shuffling and ssDNA recognition in Oxytricha nova telomere end-binding protein complexes.
Theobald et al. (2003)
1 more