› 1phh

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF P-HYDROXYBENZOATE HYDROXYLASE COMPLEXED WITH ITS REACTION PRODUCT 3,4-DIHYDROXYBENZOATE

Released:
DOI: 10.2210/pdb1phh/pdb
PDB entry 1phh coloured by chain, front view.
PDB entry 1phh coloured by chain, side view.
PDB entry 1phh coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
Crystal structure of p-hydroxybenzoate hydroxylase complexed with its reaction product 3,4-dihydroxybenzoate.
Schreuder HA, van der Laan JM, Hol WG, Drenth J
J Mol Biol 199 637-48 (1988)
PMID: 3351945

Function and Biology Details

Reaction catalysed: 
4-hydroxybenzoate + NADPH + O(2) = protocatechuate + NADP(+) + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
p-hydroxybenzoate hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 394 amino acids
Theoretical weight: 44.38 KDa
Source organism: Pseudomonas fluorescens
Expression system: Not provided
UniProt:
  • Canonical: P00438 (Residues: 1-394; Coverage: 100%)
Gene name: pobA
Sequence domains: FAD binding domain
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:
Ligand DHB 1 x DHB
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2221
Unit cell:
a: 71.9Å b: 146.3Å c: 88.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 not available not available
Expression system: Not provided

Quick links

Citations

5 review citations

Protein dynamics and electrostatics in the function of p-hydroxybenzoate hydroxylase.
Entsch et al. (2005)
4 more

7 mentions without citation

Understanding and Improving the Activity of Flavin-Dependent Halogenases via Random and Targeted Mutagenesis.
Andorfer et al. (2018)
6 more