› 1pii

X-ray diffraction
2Å resolution

THREE-DIMENSIONAL STRUCTURE OF THE BIFUNCTIONAL ENZYME PHOSPHORIBOSYLANTHRANILATE ISOMERASE: INDOLEGLYCEROLPHOSPHATE SYNTHASE FROM ESCHERICHIA COLI REFINED AT 2.0 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1pii/pdb
PDB entry 1pii coloured by chain, front view.
PDB entry 1pii coloured by chain, side view.
PDB entry 1pii coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Three-dimensional structure of the bifunctional enzyme phosphoribosylanthranilate isomerase: indoleglycerolphosphate synthase from Escherichia coli refined at 2.0 A resolution.
Wilmanns M, Priestle JP, Niermann T, Jansonius JN
J Mol Biol 223 477-507 (1992)
PMID: 1738159

Function and Biology Details

Reactions catalysed: 
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO(2) + H(2)O
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133677 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tryptophan biosynthesis protein TrpCF Chain: A
Molecule details ›
Chain: A
Length: 452 amino acids
Theoretical weight: 49.41 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P00909 (Residues: 2-453; Coverage: 100%)
Gene names: JW1254, b1262, trpC, trpF
Sequence domains:
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41
Unit cell:
a: 104.7Å b: 104.7Å c: 68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 not available not available
Expression system: Not provided

Quick links

Citations

4 review citations

Divergent evolution of enzymatic function: mechanistically diverse superfamilies and functionally distinct suprafamilies.
Gerlt et al. (2001)
3 more

24 mentions without citation

A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.
Parthasarathy et al. (2018)
23 more