› 1pix

X-ray diffraction
2.2Å resolution

Crystal structure of the carboxyltransferase subunit of the bacterial ion pump glutaconyl-coenzyme A decarboxylase

Released:
DOI: 10.2210/pdb1pix/pdb
PDB entry 1pix coloured by chain, front view.
PDB entry 1pix coloured by chain, side view.
PDB entry 1pix coloured by chain, top view.
Source organism: Acidaminococcus fermentans
Primary publication:
Crystal structure of the carboxyltransferase subunit of the bacterial sodium ion pump glutaconyl-coenzyme A decarboxylase.
Wendt KS, Schall I, Huber R, Buckel W, Jacob U
EMBO J 22 3493-502 (2003)
PMID: 12853465

Function and Biology Details

Reaction catalysed: 
4-carboxybut-2-enoyl-CoA + Na(+)(Side 1) = but-2-enoyl-CoA + CO(2) + Na(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutaconyl-CoA decarboxylase subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 587 amino acids
Theoretical weight: 64.42 KDa
Source organism: Acidaminococcus fermentans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06700 (Residues: 1-587; Coverage: 100%)
Gene names: Acfer_1817, gcdA
Sequence domains: Carboxyl transferase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand FMT 4 x FMT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P41212
Unit cell:
a: 151.869Å b: 151.869Å c: 163Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.227
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Energy conservation via electron-transferring flavoprotein in anaerobic bacteria.
Herrmann et al. (2008)
4 more

8 mentions without citation

The enzymes of biotin dependent CO₂ metabolism: what structures reveal about their reaction mechanisms.
Waldrop et al. (2012)
7 more