› 1pj9

X-ray diffraction
2Å resolution

Bacillus circulans strain 251 loop mutant 183-195

Released:
DOI: 10.2210/pdb1pj9/pdb
PDB entry 1pj9 coloured by chain, front view.
PDB entry 1pj9 coloured by chain, side view.
PDB entry 1pj9 coloured by chain, top view.
Source organism: Niallia circulans
Primary publication:
Improved thermostability of bacillus circulans cyclodextrin glycosyltransferase by the introduction of a salt bridge.
Leemhuis H, Rozeboom HJ, Dijkstra BW, Dijkhuizen L
Proteins 54 128-34 (2004)
PMID: 14705029

Function and Biology Details

Reaction catalysed: 
Cyclizes part of a (1->4)-alpha-D-glucan chain by formation of a (1->4)-alpha-D-glucosidic bond
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155054 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Cyclomaltodextrin glucanotransferase Chain: A
Molecule details ›
Chain: A
Length: 686 amino acids
Theoretical weight: 74.65 KDa
Source organism: Niallia circulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P43379 (Residues: 28-713; Coverage: 100%)
Gene name: cgt
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand GLC 1 x GLC
Ligand MPD 1 x MPD
Ligand ACY 3 x ACY
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P212121
Unit cell:
a: 117.247Å b: 109.643Å c: 65.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.152 0.176
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications.
Leemhuis et al. (2010)
3 more

3 mentions without citation

Defining structural and evolutionary modules in proteins: a community detection approach to explore sub-domain architecture.
Hleap et al. (2013)
2 more