› 1pjk

X-ray diffraction
2.5Å resolution

Crystal Structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit

Released:
DOI: 10.2210/pdb1pjk/pdb
PDB entry 1pjk coloured by chain, front view.
PDB entry 1pjk coloured by chain, side view.
PDB entry 1pjk coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of a C-terminal deletion mutant of human protein kinase CK2 catalytic subunit.
Ermakova I, Boldyreff B, Issinger OG, Niefind K
J Mol Biol 330 925-34 (2003)
PMID: 12860116

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Casein kinase II subunit alpha Chain: A
Molecule details ›
Chain: A
Length: 334 amino acids
Theoretical weight: 39.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P68400 (Residues: 2-335; Coverage: 85%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand ANP 1 x ANP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 59.044Å b: 46.502Å c: 64.262Å
α: 90° β: 112.02° γ: 90°
R-values:
R R work R free
0.188 0.18 0.257
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Protein nanocrystallography: a new approach to structural proteomics.
Pechkova et al. (2004)
38 other articles

6 mentions without citation

Substrate and docking interactions in serine/threonine protein kinases.
Goldsmith et al. (2007)
5 more