› 1pkg

X-ray diffraction
2.9Å resolution

Structure of a c-Kit Kinase Product Complex

Released:
DOI: 10.2210/pdb1pkg/pdb
PDB entry 1pkg coloured by chain, front view.
PDB entry 1pkg coloured by chain, side view.
PDB entry 1pkg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a c-kit product complex reveals the basis for kinase transactivation.
Mol CD, Lim KB, Sridhar V, Zou H, Chien EY, Sang BC, Nowakowski J, Kassel DB, Cronin CN, McRee DE
J Biol Chem 278 31461-4 (2003)
PMID: 12824176

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-145426 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mast/stem cell growth factor receptor Kit Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.55 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P10721 (Residues: 549-935; Coverage: 35%)
Gene names: KIT, SCFR
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADP 2 x ADP
1 modified residue:
Ligand PTR 4 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21212
Unit cell:
a: 92.667Å b: 116.458Å c: 60.066Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.225 0.311
Expression system: Spodoptera frugiperda

Quick links

Citations

38 review citations

Regulation of protein kinases; controlling activity through activation segment conformation.
Nolen et al. (2004)
37 more

48 mentions without citation

Mechanism of activation and the rewired network: New drug design concepts.
Nussinov et al. (2022)
47 more