› 1pl6

X-ray diffraction
2Å resolution

Human SDH/NADH/inhibitor complex

Released:
DOI: 10.2210/pdb1pl6/pdb
PDB entry 1pl6 coloured by chain, front view.
PDB entry 1pl6 coloured by chain, side view.
PDB entry 1pl6 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray crystallographic and kinetic studies of human sorbitol dehydrogenase.
Pauly TA, Ekstrom JL, Beebe DA, Chrunyk B, Cunningham D, Griffor M, Kamath A, Lee SE, Madura R, Mcguire D, Subashi T, Wasilko D, Watts P, Mylari BL, Oates PJ, Adams PD, Rath VL
Structure 11 1071-85 (2003)
PMID: 12962626

Function and Biology Details

Reactions catalysed: 
L-iditol + NAD(+) = L-sorbose + NADH
(R,R)-butane-2,3-diol + NAD(+) = (R)-acetoin + NADH
Ribitol + NAD(+) = D-ribulose + NADH
Xylitol + NAD(+) = D-xylulose + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169473 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sorbitol dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 356 amino acids
Theoretical weight: 38.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q00796 (Residues: 2-357; Coverage: 100%)
Gene name: SORD
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
2 bound ligands:
Ligand ZN 4 x ZN
Ligand 572 4 x 572
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P62
Unit cell:
a: 134.814Å b: 134.814Å c: 225.184Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.183 0.183 0.211
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
2 more

13 mentions without citation

Active site dynamics in the zinc-dependent medium chain alcohol dehydrogenase superfamily.
Baker et al. (2009)
12 more