› 1pmm

X-ray diffraction
2Å resolution

Crystal structure of Escherichia coli GadB (low pH)

Released:
DOI: 10.2210/pdb1pmm/pdb
PDB entry 1pmm coloured by chain, front view.
PDB entry 1pmm coloured by chain, side view.
PDB entry 1pmm coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase.
Capitani G, De Biase D, Aurizi C, Gut H, Bossa F, Grütter MG
EMBO J 22 4027-37 (2003)
PMID: 12912902

Function and Biology Details

Reaction catalysed: 
L-glutamate = 4-aminobutanoate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159688 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate decarboxylase beta Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 466 amino acids
Theoretical weight: 52.73 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69910 (Residues: 1-466; Coverage: 100%)
Gene names: JW1488, b1493, gadB
Sequence domains: Pyridoxal-dependent decarboxylase conserved domain
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 6 x PLP
1 bound ligand:
Ligand ACY 6 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 90.78Å b: 91.406Å c: 93.686Å
α: 76.8° β: 77.1° γ: 78.24°
R-values:
R R work R free
0.182 0.182 0.213
Expression system: Escherichia coli

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Citations

21 review citations

Escherichia coli acid resistance: tales of an amateur acidophile.
Foster JW. (2004)
20 more

9 mentions without citation

Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase.
Capitani et al. (2003)
8 more