› 1pop

X-ray diffraction
2.1Å resolution

X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX

Released:
DOI: 10.2210/pdb1pop/pdb
PDB entry 1pop coloured by chain, front view.
PDB entry 1pop coloured by chain, side view.
PDB entry 1pop coloured by chain, top view.
Source organisms:
Primary publication:
X-ray crystallographic structure of a papain-leupeptin complex.
Schröder E, Phillips C, Garman E, Harlos K, Crawford C
FEBS Lett 315 38-42 (1993)
PMID: 8416808

Function and Biology Details

Reaction catalysed: 
Hydrolysis of proteins with broad specificity for peptide bonds, but preference for an amino acid bearing a large hydrophobic side chain at the P2 position. Does not accept Val in P1'.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133545 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Papain Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.45 KDa
Source organism: Carica papaya
Expression system: Not provided
UniProt:
  • Canonical: P00784 (Residues: 134-345; Coverage: 65%)
Sequence domains: Papain family cysteine protease
Structure domains: Cysteine proteinases
LEUPEPTIN Chain: B
Molecule details ›
Chain: B
Length: 4 amino acids
Theoretical weight: 430 Da
Source organism: Streptomyces roseus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MOH 24 x MOH
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 44.92Å b: 104.96Å c: 50.97Å
α: 90° β: 90° γ: 90°
Expression system: Not provided

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Citations

7 review citations

The lysosomal cysteine proteases.
McGrath ME. (1999)
6 more

18 mentions without citation

Most efficient cocaine hydrolase designed by virtual screening of transition states.
Zheng et al. (2008)
17 more