› 1ppe

X-ray diffraction
2Å resolution

THE REFINED 2.0 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF THE COMPLEX FORMED BETWEEN BOVINE BETA-TRYPSIN AND CMTI-I, A TRYPSIN INHIBITOR FROM SQUASH SEEDS (CUCURBITA MAXIMA): TOPOLOGICAL SIMILARITY OF THE SQUASH SEED INHIBITORS WITH THE CARBOXYPEPTIDASE A INHIBITOR FROM POTATOES

Released:
DOI: 10.2210/pdb1ppe/pdb
PDB entry 1ppe coloured by chain, front view.
PDB entry 1ppe coloured by chain, side view.
PDB entry 1ppe coloured by chain, top view.
Source organisms:
Primary publication:
The refined 2.0 A X-ray crystal structure of the complex formed between bovine beta-trypsin and CMTI-I, a trypsin inhibitor from squash seeds (Cucurbita maxima). Topological similarity of the squash seed inhibitors with the carboxypeptidase A inhibitor from potatoes.
Bode W, Greyling HJ, Huber R, Otlewski J, Wilusz T
FEBS Lett 242 285-92 (1989)
PMID: 2914611

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133328 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine protease 1 Chain: E
Molecule details ›
Chain: E
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases
Trypsin inhibitor 1 Chain: I
Molecule details ›
Chain: I
Length: 29 amino acids
Theoretical weight: 3.28 KDa
Source organism: Cucurbita maxima
Expression system: Not provided
UniProt:
  • Canonical: P01074 (Residues: 1-29; Coverage: 100%)
Sequence domains: Squash family serine protease inhibitor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 59.28Å b: 55.47Å c: 74.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.151 0.151 not available
Expression system: Not provided

Quick links

Citations

12 review citations

Natural protein proteinase inhibitors and their interaction with proteinases.
Bode et al. (1992)
11 more

34 mentions without citation

The many faces of protease-protein inhibitor interaction.
Otlewski et al. (2005)
33 more