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The Crystal Structure and Reaction Mechanism of E. coli 2,4-Dienoyl CoA Reductase

Source organism: Escherichia coli
Biochemical function: FAD binding
Biological process: fatty acid beta-oxidation, unsaturated, even number, reductase/isomerase pathway
Cellular component: not assigned

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EC 1.3.1.34: 2,4-dienoyl-CoA reductase ((2E)-enoyl-CoA-producing)

Reaction catalysed:
A (2E)-2-enoyl-CoA + NADP(+) = a (2E,4E)-2,4-dienoyl-CoA + NADPH
Systematic name:
(2E)-2-enoyl-CoA:NADP(+) 4-oxidoreductase
Alternative Name(s):
  • 2,4-dienoyl-CoA reductase (NADPH)
  • 4-enoyl coenzyme A (reduced nicotinamide adenine dinucleotide phosphate) reductase
  • 4-enoyl-CoA reductase
  • 4-enoyl-CoA reductase (NADPH(2))
  • 4-enoyl-CoA reductase (NADPH)

GO terms

Sequence families

Pfam Protein families (Pfam)
PF07992
Domain description: Pyridine nucleotide-disulphide oxidoreductase
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of Pfam domain PF07992 (Pyridine nucleotide-disulphide oxidoreductase) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.

PF00724
Domain description: NADH:flavin oxidoreductase / NADH oxidase family
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of Pfam domain PF00724 (NADH:flavin oxidoreductase / NADH oxidase family) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR036188
Domain description: FAD/NAD(P)-binding domain superfamily
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
IPR023753
Domain description: FAD/NAD(P)-binding domain
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
IPR001155
Domain description: NADH:flavin oxidoreductase/NADH oxidase, N-terminal
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
IPR013785
Domain description: Aldolase-type TIM barrel
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]

Structure domains

CATH CATH domains
3.40.50.720
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: NAD(P)-binding Rossmann-like Domain
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of CATH domain 3.40.50.720 (Rossmann fold) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.
3.50.50.60
Class: Alpha Beta
Architecture: 3-Layer(bba) Sandwich
Topology: FAD/NAD(P)-binding domain
Homology: FAD/NAD(P)-binding domain
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of CATH domain 3.50.50.60 (FAD/NAD(P)-binding domain) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.
3.20.20.70
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Aldolase class I
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of CATH domain 3.20.20.70 (TIM Barrel) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.
SCOP SCOP annotations
51972
Class: Alpha and beta proteins (a/b)
Fold: Nucleotide-binding domain
Superfamily: Nucleotide-binding domain
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of SCOP domain 51972 (N-terminal domain of adrenodoxin reductase-like) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.
51906
Class: Alpha and beta proteins (a/b)
Fold: FAD/NAD(P)-binding domain
Superfamily: FAD/NAD(P)-binding domain
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of SCOP domain 51906 (C-terminal domain of adrenodoxin reductase-like) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.
51396
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: FMN-linked oxidoreductases
Occurring in:
  1. 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]
The deposited structure of PDB entry 1ps9 contains 1 copy of SCOP domain 51396 (FMN-linked oxidoreductases) in 2,4-dienoyl-CoA reductase [(2E)-enoyl-CoA-producing]. Showing 1 copy in chain A.

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