› 1ptd

X-ray diffraction
2.6Å resolution

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C

Released:
DOI: 10.2210/pdb1ptd/pdb
PDB entry 1ptd coloured by chain, front view.
PDB entry 1ptd coloured by chain, side view.
PDB entry 1ptd coloured by chain, top view.
Source organism: Bacillus cereus
Primary publication:
Crystal structure of the phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with myo-inositol.
Heinz DW, Ryan M, Bullock TL, Griffith OH
EMBO J 14 3855-63 (1995)
PMID: 7664726

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol = 1D-myo-inositol 1,2-cyclic phosphate + 1,2-diacyl-sn-glycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol phosphodiesterase Chain: A
Molecule details ›
Chain: A
Length: 298 amino acids
Theoretical weight: 34.57 KDa
Source organism: Bacillus cereus
Expression system: Escherichia coli
UniProt:
  • Canonical: P14262 (Residues: 32-329; Coverage: 100%)
Sequence domains: Phosphatidylinositol-specific phospholipase C, X domain
Structure domains: Phosphatidylinositol (PI) phosphodiesterase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 45.2Å b: 45.6Å c: 160.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.187 0.264
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Structure, function, and control of phosphoinositide-specific phospholipase C.
Rebecchi et al. (2000)
16 more

4 mentions without citation

Defining specific lipid binding sites for a peripheral membrane protein in situ using subtesla field-cycling NMR.
Pu et al. (2010)
3 more