› 1pu6

X-ray diffraction
1.64Å resolution

Crystal structure of H.pylori 3-methyladenine DNA glycosylase (MagIII)

Released:
DOI: 10.2210/pdb1pu6/pdb
PDB entry 1pu6 coloured by chain, front view.
PDB entry 1pu6 coloured by chain, side view.
PDB entry 1pu6 coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Crystal structures of 3-methyladenine DNA glycosylase MagIII and the recognition of alkylated bases.
Eichman BF, O'Rourke EJ, Radicella JP, Ellenberger T
EMBO J 22 4898-909 (2003)
PMID: 14517230

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127746 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HhH-GPD domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 218 amino acids
Theoretical weight: 25.22 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: O25323 (Residues: 1-218; Coverage: 100%)
Gene name: HP_0602
Sequence domains: HhH-GPD superfamily base excision DNA repair protein
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand BME 1 x BME
Ligand MPD 2 x MPD
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1, NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 146.605Å b: 44.399Å c: 81.516Å
α: 90° β: 106.4° γ: 90°
R-values:
R R work R free
0.153 0.151 0.185
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Base excision repair.
Krokan et al. (2013)
9 more

3 mentions without citation

Recent advances in the structural mechanisms of DNA glycosylases.
Brooks et al. (2013)
2 more