› 1puf

X-ray diffraction
1.9Å resolution

Crystal Structure of HoxA9 and Pbx1 homeodomains bound to DNA

Released:
DOI: 10.2210/pdb1puf/pdb
PDB entry 1puf coloured by chain, front view.
PDB entry 1puf coloured by chain, side view.
PDB entry 1puf coloured by chain, top view.
Source organisms:
Primary publication:
Structure of HoxA9 and Pbx1 bound to DNA: Hox hexapeptide and DNA recognition anterior to posterior.
LaRonde-LeBlanc NA, Wolberger C
Genes Dev 17 2060-72 (2003)
PMID: 12923056

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114406 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Homeobox protein Hox-A9 Chain: A
Molecule details ›
Chain: A
Length: 77 amino acids
Theoretical weight: 9.65 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09631 (Residues: 193-269; Coverage: 28%)
Gene names: Hox-1.7, Hoxa-9, Hoxa9
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
Pre-B-cell leukemia transcription factor 1 Chain: B
Molecule details ›
Chain: B
Length: 73 amino acids
Theoretical weight: 8.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P40424 (Residues: 233-305; Coverage: 17%)
Gene names: PBX1, PRL
Sequence domains: Homeodomain
Structure domains: Homeodomain-like
5'-D(*AP*CP*TP*CP*TP*AP*TP*GP*AP*TP*TP*TP*AP*CP*GP*AP*CP*GP*CP*T)-3' Chain: D
Molecule details ›
Chain: D
Length: 20 nucleotides
Theoretical weight: 6.08 KDa
5'-D(*TP*AP*GP*CP*GP*TP*CP*GP*TP*AP*AP*AP*TP*CP*AP*TP*AP*GP*AP*G)-3' Chain: E
Molecule details ›
Chain: E
Length: 20 nucleotides
Theoretical weight: 6.18 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 60.769Å b: 114.853Å c: 108.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.233 0.268
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

25 review citations

Hox cofactors in vertebrate development.
Moens et al. (2006)
24 more

40 mentions without citation

Hox specificity unique roles for cofactors and collaborators.
Mann et al. (2009)
39 more