› 1pv7

X-ray diffraction
3.6Å resolution

Crystal structure of lactose permease with TDG

Released:
DOI: 10.2210/pdb1pv7/pdb
PDB entry 1pv7 coloured by chain, front view.
PDB entry 1pv7 coloured by chain, side view.
PDB entry 1pv7 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure and mechanism of the lactose permease of Escherichia coli.
Abramson J, Smirnova I, Kasho V, Verner G, Kaback HR, Iwata S
Science 301 610-5 (2003)
PMID: 12893935

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136380 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lactose permease Chains: A, B
Molecule details ›
Chains: A, B
Length: 417 amino acids
Theoretical weight: 46.48 KDa
Source organism: Escherichia coli
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P02920 (Residues: 1-417; Coverage: 100%)
Gene names: JW0334, b0343, lacY
Sequence domains: LacY proton/sugar symporter
Structure domains: MFS general substrate transporter like domains

Ligands and Environments

Carbohydrate polymer : NEW Components: YIO, GAL
Ligand YIO 2 x YIO
Ligand GAL 2 x GAL
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 101.351Å b: 125.845Å c: 188.118Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.271 0.271 0.296
Expression system: Cricetulus griseus

Quick links

Citations

205 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
204 more

63 mentions without citation

Structural perspectives on secondary active transporters.
Boudker et al. (2010)
62 more