› 1pwy

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF HUMAN PNP COMPLEXED WITH ACYCLOVIR

Released:
DOI: 10.2210/pdb1pwy/pdb
PDB entry 1pwy coloured by chain, front view.
PDB entry 1pwy coloured by chain, side view.
PDB entry 1pwy coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of human purine nucleoside phosphorylase complexed with acyclovir.
dos Santos DM, Canduri F, Pereira JH, Vinicius Bertacine Dias M, Silva RG, Mendes MA, Palma MS, Basso LA, de Azevedo WF, Santos DS
Biochem Biophys Res Commun 308 553-9 (2003)
PMID: 12914786

Function and Biology Details

Reaction catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132560 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chain: E
Molecule details ›
Chain: E
Length: 288 amino acids
Theoretical weight: 32.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00491 (Residues: 2-289; Coverage: 100%)
Gene names: NP, PNP
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand AC2 1 x AC2
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: R32
Unit cell:
a: 139.06Å b: 139.06Å c: 160.57Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.304 0.215 0.301
Expression system: Escherichia coli

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Citations

5 review citations

Role of Marine Natural Products in the Genesis of Antiviral Agents.
Gogineni et al. (2015)
4 more

6 mentions without citation

Conformational lability in the class II MHC 310 helix and adjacent extended strand dictate HLA-DM susceptibility and peptide exchange.
Painter et al. (2011)
5 more