› 1pyy

X-ray diffraction
2.42Å resolution

Double mutant PBP2x T338A/M339F from Streptococcus pneumoniae strain R6 at 2.4 A resolution

Released:
DOI: 10.2210/pdb1pyy/pdb
PDB entry 1pyy coloured by chain, front view.
PDB entry 1pyy coloured by chain, side view.
PDB entry 1pyy coloured by chain, top view.
Source organism: Streptococcus pneumoniae R6
Primary publication:
The structural modifications induced by the M339F substitution in PBP2x from Streptococcus pneumoniae further decreases the susceptibility to beta-lactams of resistant strains.
Chesnel L, Pernot L, Lemaire D, Champelovier D, Croizé J, Dideberg O, Vernet T, Zapun A
J Biol Chem 278 44448-56 (2003)
PMID: 12923202

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157845 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Penicillin-binding protein 2X Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 76.79 KDa
Source organism: Streptococcus pneumoniae R6
Expression system: Escherichia coli
UniProt:
  • Canonical: P59676 (Residues: 49-750; Coverage: 94%)
Gene names: pbpX, spr0304
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, TRV
Ligand GLC 1 x GLC
Ligand TRV 1 x TRV
2 bound ligands:
Ligand SO4 1 x SO4
Ligand MPD 1 x MPD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: C2
Unit cell:
a: 128.224Å b: 64.746Å c: 146.974Å
α: 90° β: 118.89° γ: 90°
R-values:
R R work R free
0.244 0.218 0.244
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Penicillin-binding proteins and beta-lactam resistance.
Zapun et al. (2008)
6 more

3 mentions without citation

One ring to rule them all: Current trends in combating bacterial resistance to the β-lactams.
King et al. (2016)
2 more