› 1q45

X-ray diffraction
2Å resolution

12-0xo-phytodienoate reductase isoform 3

Released:
DOI: 10.2210/pdb1q45/pdb
PDB entry 1q45 coloured by chain, front view.
PDB entry 1q45 coloured by chain, side view.
PDB entry 1q45 coloured by chain, top view.
Source organism: Arabidopsis thaliana
Primary publication:
X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3.
Malone TE, Madson SE, Wrobel RL, Jeon WB, Rosenberg NS, Johnson KA, Bingman CA, Smith DW, Phillips GN, Markley JL, Fox BG
Proteins 58 243-5 (2005)
PMID: 15468319

Function and Biology Details

Reaction catalysed: 
8-((1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl)octanoate + NADP(+) = (15Z)-12-oxophyto-10,15-dienoate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
12-oxophytodienoate reductase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 391 amino acids
Theoretical weight: 42.74 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9FUP0 (Residues: 1-391; Coverage: 100%)
Gene names: At2g06050, DDE1, F5K7.19, OPR3
Sequence domains: NADH:flavin oxidoreductase / NADH oxidase family
Structure domains: Aldolase class I

Ligands and Environments


Cofactor: Ligand FMN 2 x FMN
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-ID-B
Spacegroup: P212121
Unit cell:
a: 78.113Å b: 85.068Å c: 121.824Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.186 0.236
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Enzymes in jasmonate biosynthesis - structure, function, regulation.
Schaller et al. (2009)
5 other articles

3 mentions without citation

Phylogenetic analysis, structural evolution and functional divergence of the 12-oxo-phytodienoate acid reductase gene family in plants.
Li et al. (2009)
2 more