› 1q78

X-ray diffraction
2.8Å resolution

Crystal structure of poly(A) polymerase in complex with 3'-dATP and magnesium chloride

Released:
DOI: 10.2210/pdb1q78/pdb
PDB entry 1q78 coloured by chain, front view.
PDB entry 1q78 coloured by chain, side view.
PDB entry 1q78 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase.
Martin G, Möglich A, Keller W, Doublié S
J Mol Biol 341 911-25 (2004)
PMID: 15328606

Function and Biology Details

Reaction catalysed: 
ATP + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150488 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A) polymerase alpha Chain: A
Molecule details ›
Chain: A
Length: 514 amino acids
Theoretical weight: 59.22 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25500 (Residues: 1-514; Coverage: 70%)
Gene names: PAP, PAPOLA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand 3AT 1 x 3AT
1 modified residue:
Ligand CSD 2 x CSD

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 57.52Å b: 63.81Å c: 180.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.255
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Protein factors in pre-mRNA 3'-end processing.
Mandel et al. (2008)
1 more

9 mentions without citation

RNA-specific ribonucleotidyl transferases.
Martin et al. (2007)
8 more