› 1q9s

X-ray diffraction
2.42Å resolution

Crystal structure of riboflavin kinase with ternary product complex

Released:
DOI: 10.2210/pdb1q9s/pdb
PDB entry 1q9s coloured by chain, front view.
PDB entry 1q9s coloured by chain, side view.
PDB entry 1q9s coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Ligand binding-induced conformational changes in riboflavin kinase: structural basis for the ordered mechanism.
Karthikeyan S, Zhou Q, Osterman AL, Zhang H
Biochemistry 42 12532-8 (2003)
PMID: 14580199

Function and Biology Details

Reaction catalysed: 
ATP + riboflavin = ADP + FMN
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188332 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Riboflavin kinase Chain: A
Molecule details ›
Chain: A
Length: 149 amino acids
Theoretical weight: 17.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q969G6 (Residues: 1-148; Coverage: 96%)
Gene name: RFK
Sequence domains: Riboflavin kinase
Structure domains: Riboflavin kinase-like

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
2 bound ligands:
Ligand MG 1 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2221
Unit cell:
a: 76.312Å b: 118.789Å c: 37.979Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.199 0.26
Expression system: Escherichia coli BL21

Quick links

Citations

5 review citations

Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers.
Abbas et al. (2011)
4 more

6 mentions without citation

Maturation of the respiratory complex II flavoprotein.
Sharma et al. (2019)
5 more