› 1qat

X-ray diffraction
3Å resolution

1-PHOSPHATIDYLINOSITOL-4,5-BISPHOSPHATE PHOSPHODIESTERASE DELTA COMPLEX WITH SAMARIUM (III) CHLORIDE

Released:
DOI: 10.2210/pdb1qat/pdb
PDB entry 1qat coloured by chain, front view.
PDB entry 1qat coloured by chain, side view.
PDB entry 1qat coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
C2 domain conformational changes in phospholipase C-delta 1.
Grobler JA, Essen LO, Williams RL, Hurley JH
Nat Struct Biol 3 788-95 (1996)
PMID: 8784353

Function and Biology Details

Reaction catalysed: 
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H(2)O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145423 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 622 amino acids
Theoretical weight: 70.43 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P10688 (Residues: 135-756; Coverage: 82%)
Gene name: Plcd1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SM 5 x SM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P1
Unit cell:
a: 61.6Å b: 75.1Å c: 86.4Å
α: 66.4° β: 85.6° γ: 89.8°
R-values:
R R work R free
0.194 0.194 0.261
Expression system: Escherichia coli

Quick links

Citations

29 review citations

The extended protein kinase C superfamily.
Mellor et al. (1998)
28 more

2 mentions without citation

Quod erat demonstrandum? The mystery of experimental validation of apparently erroneous computational analyses of protein sequences.
Iyer et al. (2001)
1 more