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THE CRYSTAL STRUCTURE OF ANTHRANILATE SYNTHASE FROM SULFOLOBUS SOLFATARICUS

X-ray diffraction
2.5Å resolution
PDB entry 1qdl coloured by chain, front view.
PDB entry 1qdl coloured by chain, side view.
PDB entry 1qdl coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Anthranilate synthase
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Multimeric state: hetero tetramer
Accessible surface area: 46403.52 Å2
Buried surface area: 7035.74 Å2
Dissociation area: 885.24 Å2
Dissociation energy (ΔGdiss): -9.14 kcal/mol
Dissociation entropy (TΔSdiss): 15.01 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-170291

Macromolecules

Anthranilate synthase component 1
Chain: A
Length: 422 amino acids
Theoretical weight: 47.78 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06128 (Residues: 1-421; Coverage: 100%)
Gene names: SSO0893, trpE
Pfam:
InterPro:
CATH: Anthranilate synthase
SCOP: ADC synthase
Anthranilate synthase component 1 in PDB entry 1qdl, assembly 1, front view.
Anthranilate synthase component 1 in PDB entry 1qdl, assembly 1, side view.
Anthranilate synthase component 1 in PDB entry 1qdl, assembly 1, top view.
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Anthranilate synthase component 2
Chain: B
Length: 195 amino acids
Theoretical weight: 21.94 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06129 (Residues: 1-195; Coverage: 100%)
Gene names: SSO0894, trpG
Pfam: Glutamine amidotransferase class-I
InterPro:
CATH: Rossmann fold
SCOP: Class I glutamine amidotransferases (GAT)
Anthranilate synthase component 2 in PDB entry 1qdl, assembly 1, front view.
Anthranilate synthase component 2 in PDB entry 1qdl, assembly 1, side view.
Anthranilate synthase component 2 in PDB entry 1qdl, assembly 1, top view.
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