› 1qib

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF GELATINASE A CATALYTIC DOMAIN

Released:
DOI: 10.2210/pdb1qib/pdb
PDB entry 1qib coloured by chain, front view.
PDB entry 1qib coloured by chain, side view.
PDB entry 1qib coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
X-ray structure of gelatinase A catalytic domain complexed with a hydroxamate inhibitor
Dhanaraj V, Williams MG, Ye Q-Z, Molina F, Johnson LL, Ortwine DF, Pavlovsky A, Rubin JR, Skeean RW, White AD, Humblet C, Hupe DJ, Blundell TL
Croatica Chemica Acta 72 575-591 (1999)

Function and Biology Details

Reaction catalysed: 
Cleavage of gelatin type I and collagen types IV, V, VII, X. Cleaves the collagen-like sequence Pro-Gln-Gly-|-Ile-Ala-Gly-Gln.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
72 kDa type IV collagenase Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 17.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08253 (Residues: 115-213, 394-449; Coverage: 25%)
Gene names: CLG4A, MMP2
Sequence domains: Matrixin
Structure domains: Collagenase (Catalytic Domain)

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand CA 3 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS
Spacegroup: P4122
Unit cell:
a: 84.83Å b: 84.83Å c: 57.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.244
Expression system: Escherichia coli

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Citations

29 mentions without citation

Application of structural dynamic approaches provide novel insights into the enzymatic mechanism of the tumor necrosis factor-alpha-converting enzyme.
Sagi et al. (2008)
28 more