› 1qj4

X-ray diffraction
1.1Å resolution

HYDROXYNITRILE-LYASE FROM HEVEA BRASILIENSIS AT ATOMIC RESOLUTION

Released:
DOI: 10.2210/pdb1qj4/pdb
PDB entry 1qj4 coloured by chain, front view.
PDB entry 1qj4 coloured by chain, side view.
PDB entry 1qj4 coloured by chain, top view.
Source organism: Hevea brasiliensis
Primary publication:
Atomic resolution crystal structure of hydroxynitrile lyase from Hevea brasiliensis.
Gruber K, Gugganig M, Wagner UG, Kratky C
Biol Chem 380 993-1000
PMID: 10494852

Function and Biology Details

Reaction catalysed: 
An aliphatic (S)-hydroxynitrile = cyanide + an aliphatic aldehyde or ketone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156604 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
(S)-hydroxynitrile lyase Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.26 KDa
Source organism: Hevea brasiliensis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P52704 (Residues: 1-257; Coverage: 100%)
Gene name: HNL
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2221
Unit cell:
a: 47.287Å b: 106.655Å c: 128.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.116 not available 0.144
Expression system: Komagataella pastoris

Quick links

Citations

7 review citations

Unveiling the functional diversity of the alpha/beta hydrolase superfamily in the plant kingdom.
Mindrebo et al. (2016)
6 more

9 mentions without citation

Structural alignment of proteins by a novel TOPOFIT method, as a superimposition of common volumes at a topomax point.
Ilyin et al. (2004)
8 more