› 1qmf

X-ray diffraction
2.8Å resolution

PENICILLIN-BINDING PROTEIN 2X (PBP-2X) ACYL-ENZYME COMPLEX

Released:
DOI: 10.2210/pdb1qmf/pdb
PDB entry 1qmf coloured by chain, front view.
PDB entry 1qmf coloured by chain, side view.
PDB entry 1qmf coloured by chain, top view.
Source organism: Streptococcus pneumoniae
Primary publication:
The crystal structure of the penicillin-binding protein 2x from Streptococcus pneumoniae and its acyl-enzyme form: implication in drug resistance.
Gordon E, Mouz N, Duée E, Dideberg O
J Mol Biol 299 477-85 (2000)
PMID: 10860753

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147020 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein 2x Chain: A
Molecule details ›
Chain: A
Length: 702 amino acids
Theoretical weight: 76.81 KDa
Source organism: Streptococcus pneumoniae
Expression system: Escherichia coli MC1061
UniProt:
  • Canonical: P14677 (Residues: 49-750; Coverage: 94%)
Gene names: SP_0336, pbpX
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CES 1 x CES
Ligand KEF 1 x KEF
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P41212
Unit cell:
a: 129.91Å b: 129.91Å c: 139.86Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.239 0.271
Expression system: Escherichia coli MC1061

Quick links

Citations

16 review citations

The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis.
Sauvage et al. (2008)
15 more

11 mentions without citation

Bacterial cell division regulation by Ser/Thr kinases: a structural perspective.
Ruggiero et al. (2012)
10 more