› 1qnl

X-ray diffraction
2.7Å resolution

AMIDE RECEPTOR/NEGATIVE REGULATOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH BUTYRAMIDE

Released:
DOI: 10.2210/pdb1qnl/pdb
PDB entry 1qnl coloured by chain, front view.
PDB entry 1qnl coloured by chain, side view.
PDB entry 1qnl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Structural adaptation to selective pressure for altered ligand specificity in the Pseudomonas aeruginosa amide receptor, amiC.
O'Hara BP, Wilson SA, Lee AW, Roe SM, Siligardi G, Drew RE, Pearl LH
Protein Eng 13 129-32 (2000)
PMID: 10708652

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aliphatic amidase expression-regulating protein Chain: A
Molecule details ›
Chain: A
Length: 385 amino acids
Theoretical weight: 42.97 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Pseudomonas aeruginosa
UniProt:
  • Canonical: P27017 (Residues: 1-385; Coverage: 100%)
Gene names: PA3364, amiC
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand BMD 1 x BMD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P42212
Unit cell:
a: 104.15Å b: 104.15Å c: 65.68Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.269 0.269 0.314
Expression system: Pseudomonas aeruginosa

Quick links

Citations

2 review citations

Cyclic-nucleotide signalling in protozoa.
Gould et al. (2011)
1 more