› 1qot

X-ray diffraction
3Å resolution

lectin UEA-II complexed with fucosyllactose and fucosylgalactose

Released:
DOI: 10.2210/pdb1qot/pdb
PDB entry 1qot coloured by chain, front view.
PDB entry 1qot coloured by chain, side view.
PDB entry 1qot coloured by chain, top view.
Source organism: Ulex europaeus
Primary publication:
Structural basis of carbohydrate recognition by lectin II from Ulex europaeus, a protein with a promiscuous carbohydrate-binding site.
Loris R, De Greve H, Dao-Thi MH, Messens J, Imberty A, Wyns L
J Mol Biol 301 987-1002 (2000)
PMID: 10966800

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190378 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Legume lectin domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 26.09 KDa
Source organism: Ulex europaeus
UniProt:
  • Canonical: Q9FVF8 (Residues: 1-242; Coverage: 94%)
Sequence domains: Legume lectin domain
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GAL, FUC, GLC
Carbohydrate polymer
3 bound ligands:
Ligand MN 4 x MN
Ligand CA 4 x CA
Ligand NAG 4 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P3221
Unit cell:
a: 104.77Å b: 104.77Å c: 175.19Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.192 0.215

Quick links

Citations

5 review citations

Norovirus disease: changing epidemiology and host susceptibility factors.
Hutson et al. (2004)
4 more

1 mention without citation

Energetics of galactose- and glucose-aromatic amino acid interactions: implications for binding in galactose-specific proteins.
Sujatha et al. (2004)