› 1qri

X-ray diffraction
2.6Å resolution

X-RAY STRUCTURE OF THE DNA-ECO RI ENDONUCLEASE COMPLEXES WITH AN E144D MUTATION AT 2.7 A

Released:
DOI: 10.2210/pdb1qri/pdb
PDB entry 1qri coloured by chain, front view.
PDB entry 1qri coloured by chain, side view.
PDB entry 1qri coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Choi J, Kim Y, Greene P, Hager P, Rosenberg JM

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114465 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Type II restriction enzyme EcoRI Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.16 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P00642 (Residues: 17-277; Coverage: 94%)
Gene name: ecoRIR
Sequence domains: Restriction endonuclease EcoRI
Structure domains: Eco RI Endonuclease, subunit A
5'-D(*TP*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3' Chain: M
Molecule details ›
Chain: M
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P321
Unit cell:
a: 118.4Å b: 118.4Å c: 49.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.164 0.164 not available
Expression system: Not provided

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