› 1qrv

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE COMPLEX OF HMG-D AND DNA

Released:
DOI: 10.2210/pdb1qrv/pdb
PDB entry 1qrv coloured by chain, front view.
PDB entry 1qrv coloured by chain, side view.
PDB entry 1qrv coloured by chain, top view.
Source organism: Drosophila melanogaster
Primary publication:
The structure of a chromosomal high mobility group protein-DNA complex reveals sequence-neutral mechanisms important for non-sequence-specific DNA recognition.
Murphy FV, Sweet RM, Churchill ME
EMBO J 18 6610-8 (1999)
PMID: 10581235

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114466 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
High mobility group protein D Chains: A, B
Molecule details ›
Chains: A, B
Length: 73 amino acids
Theoretical weight: 8.37 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05783 (Residues: 2-74; Coverage: 65%)
Gene names: CG17950, HmgD
Sequence domains: HMG (high mobility group) box
Structure domains: High mobility group box domain
DNA (5'-D(*GP*CP*GP*AP*TP*AP*TP*CP*GP*C)-3') Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C, RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 43.74Å b: 53.797Å c: 86.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.288
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

19 review citations

HMG1 and 2, and related 'architectural' DNA-binding proteins.
Thomas et al. (2001)
18 more

24 mentions without citation

The high mobility group box: the ultimate utility player of a cell.
Malarkey et al. (2012)
23 more