› 1qv1

X-ray diffraction
1.1Å resolution

Atomic resolution structure of obelin from Obelia longissima

Released:
DOI: 10.2210/pdb1qv1/pdb
PDB entry 1qv1 coloured by chain, front view.
PDB entry 1qv1 coloured by chain, side view.
PDB entry 1qv1 coloured by chain, top view.
Source organism: Obelia longissima
Primary publication:
Atomic resolution structure of obelin: soaking with calcium enhances electron density of the second oxygen atom substituted at the C2-position of coelenterazine.
Liu ZJ, Vysotski ES, Deng L, Lee J, Rose J, Wang BC
Biochem Biophys Res Commun 311 433-9 (2003)
PMID: 14592432

Function and Biology Details

Reaction catalysed: 
(1a) coelenterazine h + O(2) = coelenterazine h dioxetanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Obelin Chain: A
Molecule details ›
Chain: A
Length: 195 amino acids
Theoretical weight: 22.24 KDa
Source organism: Obelia longissima
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27709 (Residues: 1-195; Coverage: 100%)
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

5 bound ligands:
Ligand CO 2 x CO
Ligand CA 1 x CA
Ligand K 1 x K
Ligand CZH 1 x CZH
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 83.334Å b: 54.631Å c: 52.685Å
α: 90° β: 112.29° γ: 90°
R-values:
R R work R free
0.144 0.144 0.167
Expression system: Escherichia coli

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Citations

3 review citations

Coelenterazine-Dependent Luciferases as a Powerful Analytical Tool for Research and Biomedical Applications.
Krasitskaya et al. (2020)
2 more

3 mentions without citation

The crystal structures of semi-synthetic aequorins.
Toma et al. (2005)
2 more