› 1qvu

X-ray diffraction
2.96Å resolution

Crystal structure of the multidrug binding transcriptional repressor QacR bound to two drugs: ethidium and proflavine

Released:
DOI: 10.2210/pdb1qvu/pdb
PDB entry 1qvu coloured by chain, front view.
PDB entry 1qvu coloured by chain, side view.
PDB entry 1qvu coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Structural mechanism of the simultaneous binding of two drugs to a multidrug-binding protein.
Schumacher MA, Miller MC, Brennan RG
EMBO J 23 2923-30 (2004)
PMID: 15257299

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-140958 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional regulator QacR Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 194 amino acids
Theoretical weight: 23.02 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A0N4 (Residues: 1-188; Coverage: 100%)
Gene names: SAP100B_005, SAP104C_022, qacR
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ET 1 x ET
Ligand PRL 1 x PRL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P42212
Unit cell:
a: 171.8Å b: 171.8Å c: 94.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.273
Expression system: Escherichia coli

Quick links

Citations

25 review citations

Structure, function, and evolution of bacterial ATP-binding cassette systems.
Davidson et al. (2008)
24 more

4 mentions without citation

The TetR family of regulators.
Cuthbertson et al. (2013)
3 more